Monday, May 31, 2021

Freedom and Its Obligations, By Calvin Coolidge (May 30, 1924)

Freedom and Its Obligations
By Calvin Coolidge

On May 30, 1924, President Calvin Coolidge (1872–1933) offered this reflection on the meaning of Memorial Day at Arlington’s annual commemorations.

...American citizenship is a high estate. He who holds it is the peer of kings. It has been secured only by untold toil and effort. It will be maintained by no other method. It demands the best that men and women have to give. But it likewise awards to its partakers the best that there is on earth. To attempt to turn it into a thing of ease and inaction would be only to debase it. To cease to struggle and toil and sacrifice for it is not only to cease to be worthy of it but is to start a retreat toward barbarism. No matter what others may say, no matter what others may do, this is the stand that those must maintain who are worthy to be called Americans.


It is these two thoughts of union and peace which appear to me to be especially appropriate for our consideration on this day. Like all else in human experience, they are not things which can be set apart and have an independent existence. They exist by reason of the concrete actions of men and women. It is the men and women whose actions between 1861 and 1865 gave us union and peace that we are met here this day to commemorate. When we seek for the chief characteristic of those actions, we come back to the word which I have already uttered; renunciation. They gave up ease and home and safety and braved every impending danger and mortal peril that they might accomplish these ends. They thereby became in this Republic a body of citizens set apart and marked for every honor so long as our Nation shall endure. Here on this wooded eminence, overlooking the Capital of the country for which they fought, many of them repose, officers of high rank and privates mingling in a common dust, holding the common veneration of a grateful people. The heroes of other wars lie with them, and in a place of great preeminence lies one whose identity is unknown, save that he was a soldier of this Republic who fought that its ideals, its institutions, its liberties, might be perpetuated among men. A grateful country holds all these services as her most priceless heritage, to be cherished forevermore.

We can testify to these opinions, not by our words but by our actions. Our country can not exist on the renunciation of the heroic souls of the past. Public service, from the action of the humblest voter to the most exalted office, can not be made a mere matter of hire and salary. The supporters of our institutions must be inspired by a more dominant motive than a conviction that their actions are going to be profitable. We can not lower our standards to what we think will pay, but we must raise them to what we think is right. It is only in that direction that we shall find true patriotism. It is only by that method that we can maintain the rights of the individual, the sovereignty of the States, the integrity of the Union, the permanency of peace, and the welfare of mankind. You soldiers of the Republic enrolled under her banner that through your sacrifices there might be an atonement for the evils of your day. That is the standard of citizenship for all time. It is the requirement which must be met by those who hold public place. That must be the ideal of those who are worthy to share in the glory which you have given to the name of America, the ideal of those who hold fellowship with Washington and Lincoln.

Sunday, May 30, 2021

FULL INTERVIEW: WORLD RENOWNED DOCTOR BLOWS LID OFF OF COVID

 

FULL INTERVIEW: WORLD RENOWNED DOCTOR BLOWS LID OFF OF COVID 
Published May 27, 2021 
Dr. Peter McCullough, internist, cardiologist, epidemiologist, and Professor of Medicine at Texas A & M College of Medicine, Dallas, TX USA 

Peter A. McCullough, MD, MPH, FACP, FACC, FAHA, FCRSA, FCCP, FNKF, FNLAProfessor of Medicine, Texas A & M College of Medicine
Board Certified Internist and Cardiologist
President Cardiorenal Society of America
Editor-in-Chief, Reviews in Cardiovascular Medicine
Editor-in-Chief, Cardiorenal Medicine
Senior Associate Editor, American Journal of Cardiology
For more information about Dr. McCullough, please visit: heartplace.com/dr-peter-a-mcculloughPeter A. McCullough, MD, MPH, FACP, FACC, FAHA, FCRSA, FCCP, FNKF, FNLA
Professor of Medicine, Texas A & M College of Medicine
Board Certified Internist and Cardiologist
President Cardiorenal Society of America
Editor-in-Chief, Reviews in Cardiovascular Medicine
Editor-in-Chief, Cardiorenal Medicine
Senior Associate Editor, American Journal of Cardiology
For more information about Dr. McCullough, please visit: heartplace.com/dr-peter-a-mccullough

APPELLANT’S REPLY BRIEF, IN THE COURT OF APPEAL OF THE STATE OF CALIFORNIA Sixth District,THE INNS BY THE SEA, Appellant, v. CALIFORNIA MUTUAL INSURANCE CO., Appellee.

 

https://drive.google.com/file/d/1NOrJ40aTpK1xrp-se3GguWKqcPcCA5L1/view?usp=sharing 
NO. H048443
IN THE COURT OF APPEAL
OF THE STATE OF CALIFORNIA
Sixth District
THE INNS BY THE SEA
, Appellant,
v.
CALIFORNIA MUTUAL INSURANCE CO.
, Appellee.
On Appeal from the Superior Court for Monterey County
Hon. Lydia M. Villareal
Case No. 20CV001274
APPELLANT’S REPLY BRIEF


INTRODUCTION

In short, this insurance coverage case presents the issue of whether a policyholder reasonably expects the standard-form “direct physical loss of or damage to” provision in an “all risk” property insurance policy to cover business-interruption losses resulting from the “loss of or damage to” property caused by COVID-19 (and the governmental orders that followed), because COVID-19 and the orders have caused “loss” or “damage.” While the context is new, the applicable principles are not, and when they are applied correctly, the answer is “yes.”


CONCLUSION
For the reasons set forth above, Respondent cannot show (1) its interpretation is the only reasonable interpretation; and (2) even under its interpretation, the allegations would not create a possibility of coverage. As a result, Inns respectfully requests that this Court reverse the Superior Court’s judgment and remand for decision on the remaining issues or allow Inns to amend its Complaint. 

Dated: April 6, 2021

Saturday, May 29, 2021

FLAG OFFICERS 4 AMERICA: 'We are retired military leaders who pledged to support and defend the Constitution of the United States against all enemies, foreign and domestic. Although retired from active service, each of us feels bound by that oath to do what we can, in our capacity today, to protect our nation from the threats to her freedom.'

FLAG OFFICERS 4 AMERICA

Retired U.S.Generals and Admirals defending the Constitution

WHO WE ARE
We are retired military leaders who pledged to support and defend the Constitution of the United States against all enemies, foreign and domestic. Although retired from active service, each of us feels bound by that oath to do what we can, in our capacity today, to protect our nation from the threats to her freedom.


ISSUE AREAS
CHINA
BORDER CRISIS
BIG TECH
IRAN NUCLEAR DEAL
KEYSTONE PIPELINE
U.S. MILITARY

NEWS Unearthed Evidence Shows Fauci Defended ‘Integral’ Gain-Of-Function Research Despite Pandemic ‘Risks’. MAY 28, 2021 NATALIE WINTERS

 

NEWS
Unearthed Evidence Shows Fauci Defended ‘Integral’ Gain-Of-Function Research Despite Pandemic ‘Risks’.
MAY 28, 2021 NATALIE WINTERS


Writing for the American Society for Microbiology in October 2012, Fauci also posited that the risky form of research could “trigger” a pandemic:

“In an unlikely but conceivable turn of events, what if that scientist becomes infected with the virus, which leads to an outbreak and ultimately triggers a pandemic?”

“Scientists working in this field might say – as indeed I have said – that the benefits of such experiments and the resulting knowledge outweigh the risks,” he adds before alleging it is “more likely” that a pandemic would occur naturally in the paper first reported by The Australian’s Sharri Markson.

Fauci In 2012: Gain-Of-Function Research 'Worth Risk Of Lab Accident Sparking Pandemic'

Fauci In 2012: Gain-Of-Function Research 'Worth Risk Of Lab Accident Sparking Pandemic'

BY TYLER DURDEN
SATURDAY, MAY 29, 2021

America's top virologist, Anthony Fauci, argued in 2012 that the risks of a lab accident sparking a pandemic are outweighed by the potential benefits of manipulating viruses via gain-of-function research, according to previously unsurfaced remarks reported by Sharri Markson via The Australian.

 "In an unlikely but conceivable turn of events, what if that scientist becomes infected with the virus, which leads to an outbreak and ultimately triggers a pandemic?" Fauci wrote in the American Society for Microbiology in 2012, adding "Many ask reasonable questions: given the possibility of such a scenario – however remote – should the initial experiments have been performed and/or published in the first place, and what were the processes involved in this decision?""Scientists working in this field might say – as indeed I have said – that the benefits of such experiments and the resulting knowledge outweigh the risks," Fauci continued. "It is more likely that a pandemic would occur in nature, and the need to stay ahead of such a threat is a primary reason for performing an experiment that might appear to be risky."

 

The experiments are also opposed by prominent scientists, including the Cambridge Working Group of 200 researchers which issued a public warning in 2014.

“Accident risks with newly created “potential pandemic pathogens” raise grave new concerns,” the group’s letter read. “Laboratory creation of highly transmissible, novel strains of dangerous viruses, especially but not limited to influenza, poses substantially increased risks.

An accidental infection in such a setting could trigger outbreaks that would be difficult or impossible to control. Historically, new strains of influenza, once they establish transmission in the human population, have infected a quarter or more of the world’s population within two years.”

And Steven Salzberg, of the Johns Hopkins School of Medicine, in 2015 said the benefits of gain-of-function research were “minimal at best” and they could “far more safely be obtained through other avenues of research”.

“I am very concerned that the continuing gain-of-function research on influenza viruses, and more recently on other viruses, presents extremely serious risks to the public health,” he wrote.

UNITED STATES COURT OF APPEALS FOR THE SIXTH CIRCUIT ANTONIO VITOLO; JAKE’S BAR AND GRILL, LLC, Plaintiffs-Appellants, v. ISABELLA CASILLAS GUZMAN, Administrator of the Small Business Administration, Defendant-Appellee: OPINION THAPAR, Circuit Judge. This case is about whether the government can allocate limited coronavirus relief funds based on the race and sex of the applicants. We hold that it cannot.

OPINION
 _________________ 

THAPAR, Circuit Judge. This case is about whether the government can allocate limited coronavirus relief funds based on the race and sex of the applicants. We hold that it cannot. 

Thus, we enjoin the government from using these unconstitutional criteria when processing Antonio Vitolo’s application. 


It has been twenty-five years since the Supreme Court struck down the race-conscious policies in Adarand. And it has been nearly twenty years since the Supreme Court struck down the racial preferences in Gratz. As today’s case shows once again, the “way to stop discrimination on the basis of race is to stop discriminating on the basis of race.” Parents Involved, 551 U.S. at 748 (plurality opinion).

The government shall fund the plaintiffs’ grant application, if approved, before all later-filed applications, without regard to processing time or the applicants’ race or sex. The government, however, may continue to give veteran-owned restaurants priority in accordance with the law. This preliminary injunction shall remain in place until this case is resolved on the merits and all appeals are exhausted.


UNITED STATES COURT OF APPEALS
FOR THE SIXTH CIRCUIT
ANTONIO VITOLO; JAKE’S BAR AND GRILL, LLC, Plaintiffs-Appellants,
v.
ISABELLA CASILLAS GUZMAN, Administrator of the Small Business Administration, Defendant-Appellee.
Nos. 21-5517/5528
On Emergency Motion for Injunction Pending Appeal and to Expedite Appeal.
United States District Court for the Eastern District of Tennessee at Knoxville;
No. 3:21-cv-00176—Travis Randall McDonough, District Judge.
Decided and Filed: May 27, 2021
Before: NORRIS, DONALD, and THAPAR, Circuit Judges.

REFERENCE:
Appellate Court Strikes Down a Piece of Biden's Race-Based America Rescue Plan 
Three cheers to a correct ruling by the 6th Circuit Court.
MISH

Friday, May 28, 2021

The Fall of Saint Anthony Fauci & Anthony Fauci backed virus experiments ‘despite pandemic risk’

The Fall of Saint Anthony Fauci
By MICHAEL BRENDAN DOUGHERTY May 27, 2021
On the unforced errors of America’s most political doctor

If this is the last “conspiracy theory” and disinformation campaign to become transformed into the accepted wisdom — which may shortly occur — then Fauci will have to explain why it was that he accepted the role of Pandemic Hero in the first place. Or why he joined a phony and flimsy consensus that the virus had emerged in nature. Or why he so consistently downplayed the risks early on, given what he must have known or suspected the minute reports emerged of a coronavirus outbreak in Wuhan. Given that he’s already told us he lies because the public isn’t ready for the truth, how likely are his denials to be believed?



Anthony Fauci backed virus experiments ‘despite pandemic risk’
EXCLUSIVE
SHARRI MARKSON
INVESTIGATIONS WRITER
MAY 28, 2021 

Writing in the American Society for Microbiology in October 2012, Dr Fauci acknowledged the controversial scientific research could spark a pandemic.

Gain-of-function experiments – often with bat-derived coronaviruses – centre on manipulating, splicing and recombining viruses potentially into strands of highly infectious and little understood diseases.

This type of research carries such a risk of causing a pandemic that the Obama administration paused funding for gain-of-­function experiments in 22 fields in 2014, including those involving SARS, influenza and MERS.

In December 2017, the National Institute of Health, of which the NIAID is a part, announced it would resume funding the gain-of-function research.

Tuesday, May 25, 2021

UNITED STATES DISTRICT COURT DISTRICT OF MASSACHUSETTS, Dr. SHIVA AYYADURAI Plaintiff, v. WILLIAM FRANCIS GALVIN, MICHELLE K. TASSINARI, DEBRA O’MALLEY, AMY COHEN, NATIONAL ASSOCIATION OF STATE ELECTION DIRECTORS, TWITTER, INC., all in their individual capacities, and WILLIAM FRANCIS GALVIN, in his official capacity as Secretary of State for Massachusetts, Defendants. CASE No. 1:20-CV-11889-MLW

 

https://drive.google.com/file/d/1kQPRqPZl6nZ-kzr1m5cWQtIuzYkP6I1r/view?usp=sharing
VERIFIED COMPLAINT & JURY DEMAND

Introduction 

This is a Complaint to redress the Commonwealth of Massachusetts' repeated violations of Plaintiff Dr. Shiva Ayyadurai's civil rights guaranteed by the First Amendment to the United States Constitution and Article 16 of the Declaration of Rights of the Constitution of the Commonwealth of Massachusetts.

COUNT I 
Violations of the Freedom of Speech or Expression 

COUNT II 
Violations of the Right to Freedom of the Press 

COUNT III 
Violations of the Freedom to Petition the Government 

COUNT IV 
Violations of the Freedom to Peaceably Assemble 

COUNT V 
Violations of the Right to Freedom of the Press and Freedom of Speech Guaranteed by Article 16 ofthe Declaration ofRights of the Massachusetts Constitution 


PRAYER FOR RELIEF 

WHEREFORE, the Plaintiff demands that Judgment be entered against the Defendant to compensate the Plaintiff for damages, and so that justice might be upheld as the Defendant violated the Plaintiffs Constitutionally-Guaranteed Rights, applicable to such parties, and order that the following damages be awarded: 
a. Damages for Loss of Liberty; 
b. Compensatory Damages; 
c. Punitive Damages; 
d. Attorney's fees; 
e. Court costs; 
f. Pre- and post-judgment interest; 
g. Injunctive relief; or 
h. Any such other relief as this Honorable Court might deem appropriate and equitable. 

Temporarv Restraining Order 

In addition to the relief requested above, the Plaintiff requests that this Court issue a Temporary Restraining Order, restraining the Defendant from again bidding Twitter to remove, ban, or otherwise silence the Plaintiff at least until the results of the race for the U.S. Senate are announced-likely by November 4, 2020, the day after election day. 
A restraining order is necessary because: 
The Plaintiff has a substantial likelihood of success on the merits of the case, since the Office of the Secretary of the Commonwealth has admitted it silenced the Plaintiff. 
Absent an injunction, the Plaintiff would suffer an irremediable loss of rights during his campaign for the U.S. Senate. While the Plaintiff has already lost essential time and means to campaign during his race for the 2020 U.S. Senate seat, the Plaintiff would incur even more losses if the Defendant were permitted to continue to violate the Plaintiff's constitutionally-guaranteed rights; and 
Granting this restraining order will serve the public interest, as it will preserve the public's ability to hear political speech and consider a public petition. 
Granting this restraining order will no prejudice the Defendant-as no harm may come to the government being preventing from silencing peaceful political speech. 

Oct 20, 2020 COMPLAINT (Galvin) against All Defendants Filing fee: $ 400, receipt number 0101-8471903 (Fee Status: Filing Fee paid), filed by Shiva Ayyadurai.(Casieri, Daniel) (Main Document 1 replaced on 10/20/2020) (Kelly, Danielle). (Additional attachment(s) added on 10/20/2020: # 1 Civil Cover Sheet, # 2 Category Form) (Kelly, Danielle). (Entered: 10/20/2020)

Main Doc Complaint

Att 1 Civil Cover Sheet

Att 2 Category Form



   
AMENDED VERIFIED COMPLAINT
Nov 6, 2020 AMENDED COMPLAINT against William Francis Galvin, filed by Shiva Ayyadurai. (Attachments: # 1 Exhibit)(Ayyadurai, Shiva) (Entered: 11/06/2020)
Main Doc­ument Amended Complaint

Att 1 Exhibit



[PROPOSED] SECOND AMENDED VERIFIED COMPLAINT
Apr 6, 2021 AMENDED COMPLAINT PROPOSED SECOND AMENDED COMPLAINT against All Defendants, filed by Shiva Ayyadurai.(Ayyadurai, Shiva) (Entered: 04/06/2021)

Main Doc Amended Complaint


 
May 20, 2021 Motion Hearing


   
SUPPLEMENTAL MEMORANDUM
REGARDING THE ELECTIONS INTERFERENCE OPERATIONS PLAYBOOK
FOR STATE AND LOCAL OFFICIALS

THAT ESTABLISHED THE INFRASTRUCTURE, PROCESSES & PROTOCOLS
DESIGNED BY TASSINARI, COHEN, NASED, TWITTER LEGAL ET AL
IN CAMBRIDGE, MA
FOR SURVEILLANCE & REPORTING OF “THREATS” VIA
THE TWITTER TRUSTED PARTNERSHIP
AND TWITTER PARTNER SUPPORT PORTAL (PSP) PROGRAM

May 21, 2021 DECLARATION Re Influence Operations Playbook by Shiva Ayyadurai. (Attachments: # 1 Exhibit Amy Cohen Testimony, # 2 Exhibit Election Incident Coordination Guide, # 3 Exhibit Election Cybersecurity Playbook, # 4 Exhibit Election-Influence-Operations-Playbook-Part1, # 5 Exhibit Election-Influence-Operations-Playbook-Part2)(Ayyadurai, Shiva) (Entered: 05/21/2021)

Main Doc Declaration

Att 1 Exhibit Amy Cohen Testimony

Att 2 Exhibit Election Incident Coordination Guide

Att 3 Exhibit Election Cybersecurity Playbook

Att 4 Exhibit Election-Influence-Operations-Playbook-Part1

Att 5 Exhibit Election-Influence-Operations-Playbook-Part2


REFERENCE:
Ayyadurai v. Galvin (1:20-cv-11889)
District Court, D. Massachusetts


 

 

 

 

 

 

 

 

Thursday, May 20, 2021

J.D. Vance Outlines The Strategy Needed to Fight Back Against Woke Capital

 

 
May 18, 2021 
(21:21) 

REFERENCE:
THE CLAREMONT INSTITUTE CENTER FOR THE AMERICAN WAY OF LIFE

IRRESISTIBLE REVOLUTION: MARXISM’S GOAL OF CONQUEST & THE UNMAKING OF THE AMERICAN MILITARY MATTHEW LOHMEIER (2021)

IRRESISTIBLE REVOLUTION: MARXISM’S GOAL OF CONQUEST & THE UNMAKING OF THE AMERICAN MILITARY
MATTHEW LOHMEIER (2021)

DESCRIPTION
Irresistible Revolution is a timely and bold contribution from an active-duty Space Force lieutenant colonel who sees the impact of a neo-Marxist agenda at the ground level within our armed forces…



BOOK DETAILS
ISBN: 9781737067320
Publisher: Matthew L. Lohmeier
Format: Paperback
Publication Date: 5/10/2021
Page Count: 230
Category: Political Science | Political Ideologies | Communism, Post-Communism & Socialism
Language: English
Dimensions: 0.52(h) x 6"(w) x 9"(d)

REFERENCES:
May 19, 2021
Identifying Lt. Colonel Matthew Lohmeier’s real sin
By Drew Allen

In the interview, Lohmeier discussed the impact of “a neo-Marxist agenda at the ground level within our armed forces.” He condemned Critical Race Theory, explained its Marxist roots, alerted the American public to its proliferation within the American military, and warned that this agenda “will divide us; it will not unify us.” In other words, Lohmeier told the truth. This is verboten — illegal in our post-constitutional America.

Lohmeier’s words were reminiscent of the words and warnings of two great Americans -- George Washington and Abraham Lincoln; in other words, the words of an enlightened patriot and statesman.

IO 26 – Interview with Lt Col Matthew Lohmeier, USAF on CRT in DoD, His Book Irresistible Revolution
by CD Media Staff May 7, 2021

Saturday, May 15, 2021

Coronavirus Likely Originated in A Chinese Lab…But The Real Villains Are Right Here In America & “Free Speech Is A Digital Black Plague:” Bishop Garrison is Key Mercenary in Biden’s Dark War Against The First Amendment (revolver.news)

Coronavirus Likely Originated in A Chinese Lab…But The Real Villains Are Right Here In America
May 13, 2021

Lockdowns forced millions of Americans out of work. Lockdowns transformed tens of millions of Americans into neurotic wrecks afraid to go outside. Lockdowns stunted the growth of America’s children, who were otherwise at zero risk. Lockdowns gave wretched creatures like Gretchen Whitmer and Andrew Cuomo totalitarian power over people’s lives. Lockdowns enabled the total revision of America’s electoral procedures to create a new mail-in voting system that gave America the electoral integrity and transparency of a banana republic.

 

The chief crime of America’s establishment in the past year was the indefinite and useless coronavirus lockdowns. These lockdowns were not imposed by Beijing. They were imposed by lawmakers, supported by fear pornographers in the press.

It matters where the Chinese coronavirus came from. And it matters that China is an increasingly formidable rival to the U.S., economically, politically, and militarily. But what matters most of all is exposing and confronting the ruling class of America who used the coronavirus to lie to the people, hoard power for themselves, and loot the country they were supposed to serve.



“Free Speech Is A Digital Black Plague:” Bishop Garrison is Key Mercenary in Biden’s Dark War Against The First Amendment
May 12, 2021

Last week, Revolver News exposed the key hatchet man promoted to the Pentagon to install radical race theory and purge all MAGA sympathies from U.S. military organs, officers and personnel.


His name is Bishop Garrison, and he is the head of the Pentagon’s new “Countering Extremism” initiative.

And that’s the problem, isn’t it? Trump and Trump supporters are allowed to speak. That used to be called “America”. Now it’s called “Extremist”. And with the full force of the U.S. military at his back, Bishop Garrison will deign to decide the difference between the two.

So the question remains: how the hell did Bishop Garrison get selected in the first place?

Thursday, May 13, 2021

Lawrence Sellin COVID19 statement 4 May 2021 & Lawrence Sellin statement on PLA influence on US virus research programs 5 May 2021

 

 
Lawrence Sellin COVID19 statement 4 May 2021 
(7:17)

   
Lawrence Sellin statement on PLA influence on US virus research programs 5 May 2021 
(5:58)

   
How China made COVID19 by Lawrence Sellin July 12, 2021 
(0:55)

A Zika virus envelope mutation preceding the 2015 epidemic enhances virulence and fitness for transmission, Chao Shan 1 2Hongjie Xia 3Sherry L Haller 4 5 6Sasha R Azar 4 5 6Yang Liu 3Jianying Liu 4 5Antonio E Muruato 4Rubing Chen 4 5 6 7Shannan L Rossi 4 5 7Maki Wakamiya 3Nikos Vasilakis 5 7 8 9 10Rongjuan Pei 2Camila R Fontes-Garfias 3Sanjay Kumar Singh 11Xuping Xie 3Scott C Weaver 12 5 6 13 14Pei-Yong Shi 1 5 6 13 14


Abstract

Arboviruses maintain high mutation rates due to lack of proofreading ability of their viral polymerases, in some cases facilitating adaptive evolution and emergence. Here we show that, just before its 2013 spread to the Americas, Zika virus (ZIKV) underwent an envelope protein V473M substitution (E-V473M) that increased neurovirulence, maternal-to-fetal transmission, and viremia to facilitate urban transmission. A preepidemic Asian ZIKV strain (FSS13025 isolated in Cambodia in 2010) engineered with the V473M substitution significantly increased neurovirulence in neonatal mice and produced higher viral loads in the placenta and fetal heads in pregnant mice. Conversely, an epidemic ZIKV strain (PRVABC59 isolated in Puerto Rico in 2015) engineered with the inverse M473V substitution reversed the pathogenic phenotypes. Although E-V473M did not affect oral infection of Aedes aegypti mosquitoes, competition experiments in cynomolgus macaques showed that this mutation increased its fitness for viremia generation, suggesting adaptive evolution for human viremia and hence transmission. Mechanistically, the V473M mutation, located at the second transmembrane helix of the E protein, enhances virion morphogenesis. Overall, our study revealed E-V473M as a critical determinant for enhanced ZIKV virulence, intrauterine transmission during pregnancy, and viremia to facilitate urban transmission.

REFERENCE:

Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20190-20197. doi: 10.1073/pnas.2005722117. Epub 2020 Aug 3. 

Chao Shan 1 2Hongjie Xia 3Sherry L Haller 4 5 6Sasha R Azar 4 5 6Yang Liu 3Jianying Liu 4 5Antonio E Muruato 4Rubing Chen 4 5 6 7Shannan L Rossi 4 5 7Maki Wakamiya 3Nikos Vasilakis 5 7 8 9 10Rongjuan Pei 2Camila R Fontes-Garfias 3Sanjay Kumar Singh 11Xuping Xie 3Scott C Weaver 12 5 6 13 14Pei-Yong Shi 1 5 6 13 14

Affiliations

1 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555; shanchao@wh.iov.cn sweaver@UTMB.edu peshi@UTMB.edu.

2 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, China.

3 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555.

4 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555.

5 Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555.

6 Institute for Translational Science, University of Texas Medical Branch, Galveston, TX 77555.

7 Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555.

8 World Reference Center of Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX 77555.

9 Center for Biodefence and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555.

10 Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555.

11 Department of Neurosurgery-Research, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.

12 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555; shanchao@wh.iov.cn sweaver@UTMB.edu peshi@UTMB.edu.

13 Sealy Institute for Vaccine Sciences, University of Texas Medical Branch, Galveston, TX 77555.

14 Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555.

PMID: 32747564 PMCID: PMC7443865 DOI: 10.1073/pnas.2005722117